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| Cell line: | B95-8 |
| DSMZ no.: | ACC 100 |
| Species: | monkey - cotton-top tamarin (Saguinus oedipus) |
| Cell type: | lymphocytes |
| Origin: | established from peripheral blood lymphocytes of a cotton-top tamarin (cotton-top marmoset) monkey (Saguinus oedipus); cells were described in the literature to release high titers of Epstein-Barr virus (EBV) providing a source for transformation of B cells and establishment of continuous cell lines |
| Reference(s): | 14764, 14765 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions: | A, B, E |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round to oval cells or clustered in small clumps in suspension and partially adherent; ca. 10% adhere, but trypsin is not necessary to detach the cells; image; |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:5 every 72 hours; minimal density at 0.2 x 106 cells/ml; adherent cells might be detached by tapping the flask; seed out at ca. 0.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 41 hours |
| Harvest: | maximal density at about 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Saguinus oedipus species. |
| Cytogenetics: | near-diploid simian karyotype with 4% polyploidy - 44(42-47)<2n> - chromosome rearrangements present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
