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Cell line: | B95-8 |
DSMZ no.: | ACC 100 |
Species: | monkey - cotton-top tamarin (Saguinus oedipus) |
Cell type: | lymphocytes |
Origin: | established from peripheral blood lymphocytes of a cotton-top tamarin (cotton-top marmoset) monkey (Saguinus oedipus); cells were described in the literature to release high titers of Epstein-Barr virus (EBV) providing a source for transformation of B cells and establishment of continuous cell lines |
Reference(s): | 14764, 14765 |
Biosafety level: | 2 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
Permissions and restrictions: | A, B, E |
| DSMZ Cell Culture Data: |
Morphology: | single, round to oval cells or clustered in small clumps in suspension and partially adherent; ca. 10% adhere, but trypsin is not necessary to detach the cells; image; |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:5 every 72 hours; minimal density at 0.2 x 106 cells/ml; adherent cells might be detached by tapping the flask; seed out at ca. 0.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 41 hours |
Harvest: | maximal density at about 3 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Saguinus oedipus species. |
Cytogenetics: | near-diploid simian karyotype with 4% polyploidy - 44(42-47)<2n> - chromosome rearrangements present |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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