| | |
|---|
| Cell line: | BC3H1 |
| DSMZ no.: | ACC 171 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | muscle cells from intracranial tumor |
| Origin: | established from the offspring of a C3H-mouse injected with nitrosoethylurea during pregnancy; cells show properties characteristic of muscle cells and were described in the literature to synthesize adenylate and creatine phosphokinases, tropomyosin and myosin |
| Reference(s): | 14946, 16746 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, flat cells forming a confluent monolayer; image; image;image |
| Medium: | 80% DMEM + 20 % h.i. FBS |
| Subculture: | split confluent culture 1:2 to 1:3 every week (cells grow very slowly) using trypsin/EDTA; cell numbers may be difficult to ascertain due to formation of clumps after detachment |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70 hours |
| Harvest: | about 1-2 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near-tetraploid karyotype - 74-82<4n> - 1-3 centric fusion markers and 1-2 dm present in most cells - does not correspond with published karyotype of 56 chromosomes |
| Viruses: | ELISA: reverse transcriptase positive |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
