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Cell line: | BE-13 (subclone of PEER) |
DSMZ no.: | ACC 396 |
Species: | human (Homo sapiens) |
Cell type: | T cell leukemia |
Origin: | tetraploid subclone of cell line PEER (DSMZ ACC 6); see there |
Reference(s): | 14884, 14574, 14608, 15867, 15868, 15217 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single round cells growing in suspension; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at about 1 x 106 cells/ml in a 24- or 6-well plate; maintain at 0.5-2.0 x 106 cells/ml; split 1:2 to 1:3 every 2-4 days; (cells grow clearly better in 24- or 6-well plates than in flasks) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40-60 hours |
Harvest: | maximal density at ca. 2.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to PEER |
Cytogenetics: | human flat-moded hypotetraploid karyotype - 81-89<4n>XXXX, +1, +4, -9, -9, -10, -16, -21, del(1)(q13.2), add(1)(p11-21), del(4)(q11q31.2)/i(4p)x2, i(4q)del(4q31.2)/i(4q)add(4)(q31)x2, del(5)(q14q21)x2, del(6)(q22)x2, del(9)(p22)x2, der(17)t(17;?21)(p11;q11)x2 - 5q- and apparent 9p22-pter nullisomy |
Molec. Genetics: | expression of fusion gene NUP214-ABL1 confirmed by RT-PCR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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