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| Cell line: | BEWO |
| DSMZ no.: | ACC 458 |
| Species: | human (Homo sapiens) |
| Cell type: | choriocarcinoma |
| Origin: | a human choriocarcinoma sample was taken at autopsy from a cerebral metastasis; cells were initially transplanted to the cheek pouch of a hamster and maintained by serial passage for 8 years prior to growth in culture vessels |
| Reference(s): | 14607, 14836, 14837, 14543, 40410 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial-like cells growing as monolayer; image; image |
| Medium: | 85-90% Ham's F12 + 10-15% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:4 - 1:6 every 3-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypertriploid karyotype with 24% polyploidy - 78(77-80)<3n>XXYY, +1, +2, +5, +5, +6, +8, +12, -13, +14, -18, +19, -20, +21, +4-6mar, +0-2 dmin, add(X)(p21-22), del(X)(p11), add(1)(p36), der(1)t(1;1)(p3?6;q2?1), i(13q), add(18)(q2?), der(19)t(9;19)(q13;p13), del(22)(q12) - sideline with del(3)(p12p23) - resembles published karyotype and (marginally) that of JEG-3 (DSM ACC 463) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
