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Cell line: | BF-32 |
DSMZ no.: | ACC 272 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | hybridoma (anti-rat fiber/myosin) |
Origin: | described as secreting a mouse monoclonal antibody (IgM) reacting with type I and IIA fibers in immunofluorescence and with type 1 and 2A myosin heavy chains (MHCs) in Western blotting; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine fetal muscles |
Reference(s): | 14929 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | grows as single cells or in clumps in suspension; loosely adherent at times; image; image; image |
Medium: | 90% DMEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
Subculture: | split saturated culture at 1:2 to 1:3 every 2-4 days |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | about 34 hours |
Harvest: | maximal density at about 1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% horse serum, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Immunology: | secretion of a IgM monoclonal antibody |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine hypotetraploid karyotype with 4.5% polyploidy - 72-80<4n> - 2-4 dms present in most cells |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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