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Cell line: | BF-45 |
DSMZ no.: | ACC 221 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | hybridoma (anti-rat fiber/myosin) |
Origin: | described as secreting a mouse monoclonal antibody (IgG1) that reacts with rat embryonic fibers in immunofluorescence and with embryonic myosin heavy chains in Western blotting; established by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles |
Reference(s): | 14928 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells); image; image |
Medium: | 90% DMEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
Subculture: | seed out at ca. 0.25 x 106 cells/ml; split saturated culture at 1:3 to 1:4 every 2 days; viability may be low after thawing, but cells will recover soon |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 45 hours |
Harvest: | maximal density at about 1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% horse serum, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | secretion of a IgG1 monoclonal antibody |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine flat-moded hypertetraploid karyotype with 5% polyploidy - 78-93<4n> - 1-2 dmin present in most cells |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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