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| Cell line: | BHK-21 |
| DSMZ no.: | ACC 61 |
| Species: | Syrian hamster (golden hamster) (Mesocricetus auratus) |
| Cell type: | kidney |
| Origin: | BHK-21 is a subclone (clone 13) of the parental line established from the kidneys of five unsexed, one-day-old hamsters in 1961; cells can be used for virus replication studies |
| Reference(s): | 14727, 14726 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblastoid adherent monolayer; image; image; image |
| Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + 1x non-essential amino acids |
| Subculture: | seed out at ca. 1-1.5 x 106 cells/ 80 cm2;
split confluent culture 1:4 to 1:10 every 2-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 25-50 hours |
| Harvest: | about 10-15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mesocricetus auratus species. |
| Cytogenetics: | near-diploid golden (Syrian) hamster karyotype with 21% tetraploidy - 40-45, XY - hyperdiploid sideline present at 19% - resembles published description |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
