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Cell line: | BL-65 |
DSMZ no.: | ACC 920 |
Species: | human (Homo sapiens) |
Cell type: | Burkitt lymphoma |
Origin: | established in 1982 from a 3-year-old girl with Burkitt lymphoma; cells are EBV-positive and were described to carry a t(8;14) translocation; a matched B-lymphoblastoid cell line is available (IARC-305, DSMZ ACC 928) |
Reference(s): | 40226 |
Biosafety level: | 2 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
Permissions and restrictions: | A, B |
| DSMZ Cell Culture Data: |
Morphology: | mostly round cells growing singly, partly in aggregates in suspension; image |
Medium: | 85-90% Iscove's MDM + 10-15% h.i. FBS + 100 µM Thioglycerol |
Subculture: | seed out at about 0.5 x 106 cells/ml; split
1:4 to 1:10 every 2-4 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 30-40 hours |
Harvest: | maximal density of ca. 1.8 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD30 -, CD34 -, CD37 +, CD38 +, cy CD79a +, CD138 -, HLA-DR +, cy IgM +, cy IgG -, cy kappa -, cy lambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching autologous IARC-305 |
Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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