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| Cell line: | BL-67 |
| DSMZ no.: | ACC 921 |
| Species: | human (Homo sapiens) |
| Cell type: | Burkitt lymphoma |
| Origin: | established from ascites fluid of a 35-year old female with EBV-positive Burkitt lymphoma; desribed to carry t(14;18) chromosomal translocation, resulting in IgH-MYC fusion transcripts |
| Reference(s): | 14714, 40212, 40213, 40214, 40226, 40301, 40302 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions: | A, B |
| | DSMZ Cell Culture Data: |
| Morphology: | small, mostly round cells growing singly in suspension and in small clumps; image; image; image |
| Medium: | 90% Iscove's MDM + 10% h.i. FBS + 100 µM Thioglycerol |
| Subculture: | seed out at 0.1 - 0.5 x 106 cells/ml; split
1:4 to 1:10 every 2-5 days; optimal density 0.3 - 1.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 10-25 hours |
| Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cy IgM +, sm/cykappa -, sm/cy lambda +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
