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Cell line: | BL-74 |
DSMZ no.: | ACC 922 |
Species: | human (Homo sapiens) |
Cell type: | Burkitt lymphoma |
Origin: | established from the ascites of a 33-year old woman with sporadic Burkitt lymphoma after relapse; cells are EBV+ and described to carry a t(8;14) translocation leading to MYC-IGH fusion, a third chromosome 10 and a homozygous deletion at 6q26-q27 (D6S347); a matched B-lymphoblastoid cell line is available (IARC-290A, DSMZ ACC 929) |
Reference(s): | 40251, 14714, 40226, 14434, 40292 |
Biosafety level: | 2 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2. |
Permissions and restrictions: | A, B |
| DSMZ Cell Culture Data: |
Morphology: | mostly round cells growing singly and partly in small clusters in suspension; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
Subculture: | seed out at 0.3-0.5 x 106 cells/ml; split 1:3 to 1:4 every 2-3 days and maintain at 0.5-1 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 45 hours |
Harvest: | ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD30 +, CD34 -, CD38 +, CD80 +, CD138 -, HLA-DR +, cy kappa +, cy lambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, , matching autologous IARC-290A |
Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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