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| Cell line: | BLN-3 |
| DSMZ no.: | ACC 787 |
| Species: | human (Homo sapiens) |
| Cell type: | glioblastoma |
| Origin: | established in 2013 from an intraoperative glioblastoma specimen |
| Reference(s): | 18246 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | most cells grow adherently like neuronal cells in monolayer, some cells grow loosely attached in clumps;
image; image; image |
| Medium: | Neurobasal medium + 0.5x B-27 supplement with vitamin A, 0.5x N-2 supplement, 20 ng/ml bFGF, 20 ng/ml EGF, 2 mM L-glutamine; omit FBS! |
| Subculture: | seed out at ca. 1.0 x 106 cells in an 80 cm2 flask; cells grow slowly after thawing; split culture 1:5 to 1:10 every 5-8 days with trypsin/EDTA (cells can be detached by tapping the flask or by pipetting) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 2-4 days |
| Harvest: | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage: | frozen with 90% medium, 10% DMSO (no FBS added) |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
