| | |
|---|
| Cell line: | BT-474 |
| DSMZ no.: | ACC 64 |
| Species: | human (Homo sapiens) |
| Cell type: | breast ductal carcinoma |
| Origin: | established from the primary tumor of an invasive ductal carcinoma of the breast obtained from a 60-year-old woman; cells were reportedly tumorigenic in athymic mice and were found to be susceptible to mouse mammary tumor virus; described to carry a mutation in the TP53 gene, rendering cells temperature sensitive; listed in the CCLE (ref 18232); RNA sequence data are available (see Ref 40906 and RNA-Seq) |
| Reference(s): | 14706, 18259, 18232, 18234, 40906 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent patches of epithelial cells; individual colonies with a thick center and a thin smooth margin; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 10 µg/ml human insulin + 2 mM L-glutamine |
| Subculture: | seed out at ca. 1-3 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 7-10 days using trypsin/EDTA; cells grow slowly and may produce cell debris |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 100 hours |
| Harvest: | about 1.0-1.6 x 105 cells/cm2 or 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent (MRA), thereafter negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypertetraploid karyotype - 104(91-111)<4n> |
| Molec. Genetics: | expression of ER, PR, AR and HER2 determined by Western blot |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
