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Cell line: | BXPC-3 |
DSMZ no.: | ACC 760 |
Species: | human (Homo sapiens) |
Cell type: | pancreas carcinoma |
Origin: | established in 1980 from the biopsy specimen of the primary adenocarcinoma of the body of the pancreas from a 61-year-old woman; cells were described not to express cystic fibrosis transmembrane conductance regulator (CFTR) |
Reference(s): | 18730, 18731 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like cells growing adherently as monolayer in tight clusters or islands; single cells sometimes with long extensions; inside the nuclei distinct spots are visible; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
Subculture: | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:2 - 1:4 every 2-3 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35-45 hours |
Harvest: | cell harvest of ca. 10 x 106 cells/75 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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