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| Cell line: | C2C12 |
| DSMZ no.: | ACC 565 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | myoblasts |
| Origin: | subclone of a mouse myoblast cell line established from mouse strain C3H; C2C12 cells are described in the literature to differentiate rapidly forming myotubes when incubated with horse serum and to differentiate into osteoblasts after treatment with bone morphogenic protein 2 |
| Reference(s): | 14393, 14651 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | polymorphic cells growing adherently in monolayers; image; image; image; image |
| Medium: | 90% DMEM (4.5 g/l glucose) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out 1-2 x 105 cells in a 75 cm2 flask; at 50-60% confluency split culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA (do not allow cultures to become confluent); Induction of differentiation with no/low FBS but 2% HS (Differentiation
protocol
) |
| Incubation: | at 37 °C with 5-10% CO2 |
| Doubling time: | ca. 12-24 hours |
| Harvest: | cell harvest of ca. 2-3 x 106 cells/75 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine flat moded near-tetraploid karyotype |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
