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| Cell line: | C6-BU-1 |
| DSMZ no.: | ACC 108 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | brain tumor |
| Origin: | this clone is a bromodeoxyuridine-resistant mutant derived from cell line C6 (established from a nitrosourea-induced brain tumor in a Wistar-Furth strain rat in 1971) |
| Reference(s): | 14598, 14597, 14355, 14380 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | spindle-shaped, relatively small cells, growing adherently in monolayers; image; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + 100 µM 5-bromodeoxyuridine |
| Subculture: | split confluent cultures 1:4 to 1:10 every 4 days using trypsin/EDTA; let the cells not grow to complete confluence; seed out at ca. 4 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | about 30 hours |
| Harvest: | about 2-3 x 107 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
