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| Cell line: | CA-46 |
| DSMZ no.: | ACC 73 |
| Species: | human (Homo sapiens) |
| Cell type: | Burkitt lymphoma |
| Origin: | established from the ascites fluid of a patient with American-type Burkitt lymphoma; cells were described in the literature to be EBNA-negative, to carry the t(8;14) and to express BCL2 and MYC mRNA |
| Reference(s): | 14730, 14731 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly round, some oval or pear-like cells growing singly in suspension; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at 0.5 x 106 cells/ml; maintain at 0.5-2.0 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 35 hours |
| Harvest: | saturation density more than 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD10+, CD13-, CD19+, CD20(+), CD34-,
CD37+, CD38+, CD80(+), CD138(+), HLA-DR+,
cyCD79a+, sIgG-, sIgM+, sIg-kappa+, sIg-lambda-,
cIgG-, cIgM+, cIg-kappa+, cIg-lambda; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near diploid karyotype with about 3% triploidy - 46(45-48)<2n>X/XY, dup(1)(q21q32), dup(7)(q12q22), t(8;14)(q24;q32) - additional rearrangements were present in a sideline, viz. t(6;13)(p21;q32) and del(11)(p11) - a supernumerary dmin was present in about 25% metaphases |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
