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Cell line: | CADO-ES1 |
DSMZ no.: | ACC 255 |
Species: | human (Homo sapiens) |
Cell type: | Ewing's sarcoma |
Origin: | established from the malignant pleural effusion from a 19-year-old woman diagnosed with right-buttock Ewing's sarcoma with multiple lung metastases; described to be heterotransplantable, capable of anchorage-independent growth and able to neural differentiation in response to cAMP |
Reference(s): | 14675, 14676 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round or fusiform cells growing in clumps as adherent or semi-adherent multicellular spheroids; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 2-3 x 106 cells in a 80 cm2 flask; split confluent culture 1:4 to 1:10 every 3-7 days using trypsin/EDTA; after thawing use 20% FBS at first |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of about 30 x 106 cells/80cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 8% polyploidy - 47(44-48)<2n>XX, +8, dup(1)(q32q44), i(8q), add(18)(p11.32) - t(11;22) absent - corresponds to published karyotype - absence of EWS-FLI1 fusion transcripts confirmed by RT-PCR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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