| | |
|---|
| Cell line: | CAL-33 |
| DSMZ no.: | ACC 447 |
| Species: | human (Homo sapiens) |
| Cell type: | tongue squamous cell carcinoma |
| Origin: | established from the surgically removed fragment of a tongue lesion from a 69-year-old man with moderately differentiated squamous cell carcinoma of the tongue in 1983 (prior to therapy); described to be tumorigenic in nude mice; listed in the CCLE (ref 18232) with genomic and protein analyses |
| Reference(s): | 14562, 18232, 40405 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherent in monolayers; image; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at 2-4 x 106 cells/175 cm2; split confluent culture 1:3 to 1:5 every 4-5 days using trypsin/EDTA (for about 10 min) |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | cell harvest of ca. 40 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy 49(45-49)<2n>XY/XYY, +7, +9, -18, +20, +mar, add(X)(p22), add(3)(p22-24), i(7p), i(8q), add(9)(p24), del(9)(p2?2) - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
