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| Cell line: | CAL-51 |
| DSMZ no.: | ACC 302 |
| Species: | human (Homo sapiens) |
| Cell type: | breast carcinoma |
| Origin: | established from the pleural effusion metastasis of a 45-year-old woman with progressive ductal breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype; described as triple-negative cell line; listed in the CCLE (ref 18232);
RNA sequence data are available (see Ref 40906 and RNA-Seq) |
| Reference(s): | 14563, 40250, 18232, 40608, 40906 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris; image; image; image |
| Medium: | 80-90% DMEM (4.5 g/L glucose) + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split culture 1:2 to 1:4 once or twice a week using trypsin/EDTA before cells reach complete confluency |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | 24-30 hours |
| Harvest: | cell harvest of ca. 15-20 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype |
| Molec. Genetics: | triple-negative confirmed by absence of ER, PR and weak HER2 expression in Western blots |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
