| | |
|---|
| Cell line: | CAL-54 |
| DSMZ no.: | ACC 365 |
| Species: | human (Homo sapiens) |
| Cell type: | kidney carcinoma |
| Origin: | established from the malignant pleural effusion of a 75-year-old man with kidney carcinoma in 1987 |
| Reference(s): | 14566 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | variously shaped, adherent cells growing in monolayers; image; image; image; image |
| Medium: | 85% DMEM + 15% h.i. FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 0.4 µg/ml hydrocortisone + 10 ng/ml EGF |
| Subculture: | split nearly confluent culture 1:2 to 1:3 about once a week using trypsin/EDTA (it may be difficult to detach the cells from the flask - use double quantity of trypsin/EDTA for ca. 15 min); seed out at ca. 2 x 106 cells/75 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 72 hours |
| Harvest: | cell harvest of ca. 8 x 106 cells/75 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 12% polyploidy - 52(52-53)<2n>X, -Y, +2, +3, +7, +7, +8, +12, -13, +15, +16, +17, del(3)(p21), del(5)(p14.2p15.2), dup(7)(q11q22), add(14)(p12) - del(3p), gain of ch 7 and loss of Y typical of renal cell carcinoma |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
