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| Cell line: | CAL-78 |
| DSMZ no.: | ACC 449 |
| Species: | human (Homo sapiens) |
| Cell type: | chondrosarcoma |
| Origin: | established from the recurrence of a dedifferentiated chondrosarcoma (grade III) of the muscle of the thigh from a 76-year-old man in 1989; tumorigenic chondrosarcomatous differentiation of xenografted tumor |
| Reference(s): | 15150 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblast-like cells growing as monolayer; the culture contains a large amount of cellular debris; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/25 cm2; cells grow extremely slowly; split confluent culture 1:2 every 10-12 days using trypsin with EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 240 hours |
| Harvest: | cell harvest of ca. 10-14 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
