| | |
|---|
| Cell line: | CAPAN-1 |
| DSMZ no.: | ACC 244 |
| Species: | human (Homo sapiens) |
| Cell type: | pancreatic ductal adenocarcinoma |
| Origin: | established from the liver metastasis of a pancreatic ductal adenocarcinoma in a 40-year-old man in 1974; described as growing in nude mice forming metastases, as resistant to 5-FU, secreting cytokines and to express cystic fibrosis transmembrane conductance regulator (CFTR) and to carry mutations in KRAS, SMAD4 and TP53 |
| Reference(s): | 15198, 14538, 18731, 18168, 40710, 40728 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblastic semi-confluent monolayer forming loosely adherent foci; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split 1:2 to 1:4 every 6-10 days using trypsin/EDTA; after thawing, viability may drop below 60% temporarily and initial growth could be slow |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 50-100 hours |
| Harvest: | cell harvest of about 1.5 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | apparently human karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
