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Cell line: | CCC-5 |
DSMZ no.: | ACC 810 |
Species: | human (Homo sapiens) |
Cell type: | cholangiocellular carcinoma |
Origin: | established in 2015 from the malignant pleural effusion of a distal cholangiocellular carcinoma of a 59-year-old man; cells were described in the literature to carry several mutations in oncogenes and tumor suppressor genes (see ref.) |
Reference(s): | 18142 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently in monolayers in a cobblestone pattern; image; image |
Medium: | 80% mixture of DMEM and Keratinocyte SFM (at 2:1) + 20% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split semi-confluent culture 1:2 to 1:5 once or twice a week using trypsin/EDTA or TrypLE Ex for about 10 min (cells are difficult to detach; rinse 2x with PBS before adding trypsin) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 2-3 days |
Harvest: | cell harvest of 5-8 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Plasmocin, then negative in PCR assay |
Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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