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| Cell line: | CCLF_MELM_0026_T_B |
| DSMZ no.: | ACC 935 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established in 2021 from a brain metastasis of a 58-year old woman with untreated malignant melanoma; a sister cell line, CCLF_MELM_0026_T_A, exists (Internal CCLF ID: CCLF_KL1412T) |
| Reference(s): | 40737 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epithelial-like cells growing in monolayers; image; image |
| Medium: | AR5 medium after SOP CELL301-AR5 but without antibiotics |
| Subculture: | seed out at about 0.5 x 106 cells in a T75 flask; split at about 80% confluency 1:2 to 1:3 every 3-6 days (rinse culture 2x with PBS and use prewarmed TrypLE Express) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 3 days |
| Harvest: | maximum density of ca. 1 x 106 cells/T25 flask |
| Storage: | frozen with RecoveryTM Cell Culture Freezing Medium |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Molec. Genetics: | BRAF K601E homozygous, IL7R V138I homozygous, TP53 T211I homozygous; data according to gene panel analysis at CCLF, verified by RNA-seq analysis at DSMZ |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 900,- € | Growing culture
(please inquire for exact delivery time) | | 1800,- € | | DNA isolated from cell line (25 µg) | | 1120,- € | | DNA isolated from cell line (5 µg) | | 280,- € |
see price list |
| | Print data sheet |
