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| Cell line: | CCLF_NEURO_0010_T |
| DSMZ no.: | ACC 930 |
| Species: | human (Homo sapiens) |
| Cell type: | spindle cell sarcoma |
| Origin: | established in 2016 from a brain metastasis of 27-year-old man with a pre-treated soft tissue spindle cell sarcoma (site of primary tumor unknown) |
| Reference(s): | 40737 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblastoid adherent cells growing in aggregates; cells contain significant cytoplasmatic vesicles; image; image; image; image; image |
| Medium: | the cells are cultivated serum-free in NSA medium after SOP CELL307-NSA but without antibiotics |
| Subculture: | seed out at ca. 1-2 x 106 cells in a T75 flask coated with laminin; split saturated culture 1:2 to 1:8 every 3-8 days with Accutase (up to 10 min; wash cells with D-PBS before) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-80 hrs |
| Harvest: | maximum density of ca. 1.4 x 105 cells/cm2 |
| Storage: | frozen with RecoveryTM Cell Culture Freezing Medium |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Species validation: | human |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 900,- € | Growing culture
(please inquire for exact delivery time) | | 1800,- € | | DNA isolated from cell line (25 µg) | | 1120,- € | | DNA isolated from cell line (5 µg) | | 280,- € |
see price list |
| | Print data sheet |
