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Cell line: | CCLF_NEURO_0048_T |
DSMZ no.: | ACC 939 |
Species: | human (Homo sapiens) |
Cell type: | glioblastoma |
Origin: | established in 2018 from an untreated glioblastoma of a 59-year old man |
Reference(s): | 40737 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | grows in neurospheres, single or fused to larger aggregates (spheroids); image; image; image |
Medium: | the cells are cultivated serum-free in NS-A medium + 20 ng/ml EGF + 20 ng/ml FGF2 + 10 µg/ml Heparin, modified SOP CELL307-NSA but without antibiotics |
Subculture: | cells are difficult to culture; cultivate in low-adherent 6-well plates; seed out all cells (approx 2 x 106 cells) into 1 well with about 4 ml medium; change/add medium when turning into orange-yellow or at least once a week; disperse aggregates gently 1-2 times a week and only when a size of about 0.5-1 mm is reached (use pipettes with wide opening (serological, wide-bore or cut); optimal density 6-10 large spheroids/well. Culture plates should not be used longer than 4 weeks |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 8-10 days |
Harvest: | 6-10 large spheroids in one well of a 6-well may contain up to 2 x 106 cells |
Storage: | frozen with RecoveryTM Cell Culture Freezing Medium |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | NF1 R440* heterozygous, PIK3R1 K567E heterozygous; data according to gene panel analysis at CCLF, verified by RNA-seq analysis at DSMZ |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 900,- € | Growing culture (please inquire for exact delivery time) | | 1800,- € | DNA isolated from cell line (25 µg) | | 1120,- € | DNA isolated from cell line (5 µg) | | 280,- € | see price list |
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