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Cell line: | CCLF_NEURO_0107_T |
DSMZ no.: | ACC 940 |
Species: | human (Homo sapiens) |
Cell type: | glioblastoma |
Origin: | established in 2017 from a biopsy of a recurrent tumor in the brain of a 55-year-old woman with glioblastoma; |
Reference(s): | 40737 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | neuronal-like elongated cells growing adherently in monolayers; image; image; image |
Medium: | the cells are cultivated serum-free in NSA medium after SOP CELL307-NSA but without antibiotics |
Subculture: | use laminin-coated flasks and seed out at about 0.5 x 106 cells in a T25 flask or 1 x 106 cells in a T75 flask; split confluent culture 1:2 every 3-5 days using warm TrypLE Express, after rinsing the cell layer with PBS; change medium every 3 - 5 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 4 days |
Harvest: | maximum density of ca. 2 x 106 cells/T25 flask |
Storage: | frozen with RecoveryTM Cell Culture Freezing Medium |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
Molec. Genetics: | RB1 L389*homozygous, TP53 R237H homozygous; data according to gene panel analysis at CCLF, verified by RNA-seq analysis at DSMZ |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 900,- € | Growing culture (please inquire for exact delivery time) | | 1800,- € | DNA isolated from cell line (25 µg) | | 1120,- € | DNA isolated from cell line (5 µg) | | 280,- € | see price list |
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