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| Cell line: | CCLF_PEDS_0008_T |
| DSMZ no.: | ACC 969 |
| Species: | human (Homo sapiens) |
| Cell type: | epithelioid sarcoma |
| Origin: | established in 2015 from the pleural cavity of a 17-year old woman with a pretreated metastatic epithelioid sarcoma |
| Reference(s): | 40737 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing in monolayers; image; image; image |
| Medium: | SMGM medium after SOP CELL312-SMGM but without antibiotics |
| Subculture: | seed out at about 1 x 106 cells in a T75 flask; split 1:2 to 1:4 every 2-4 days (rinse culture 2x with PBS and use TrypLE Express) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 2-3 days |
| Harvest: | maximum density of 2-3 x 106 cells/T75 flask |
| Storage: | frozen with RecoveryTM Cell Culture Freezing Medium |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Molec. Genetics: | CDKN2A A148T heterozygous; data according to gene panel analysis at CCLF, verified by RNA-seq analysis at DSMZ and loss of CDH1 and SMARCB1 gene expression (DSMZ RNA-seq data) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 900,- € | Growing culture
(please inquire for exact delivery time) | | 1800,- € | | DNA isolated from cell line (25 µg) | | 1120,- € | | DNA isolated from cell line (5 µg) | | 280,- € |
see price list |
| | Print data sheet |
