| | |
|---|
| Cell line: | CCLF_PEDS_0051_T |
| DSMZ no.: | ACC 972 |
| Species: | human (Homo sapiens) |
| Cell type: | neuroblastoma |
| Origin: | established in 2021 from the pleural cavity of a 2-year-old boy diagnosed with untreated metastatic neuroblastoma (Internal CCLF ID: PEDS195T_4_PF) |
| Reference(s): | 40737 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells displaying two different morphologies with varying ratio: neuronal-like cells with long extensions and large flat epitheloid and probably senescent cells; image; image; image; image; image; image; image; image; image; image |
| Medium: | 50% Advanced DMEM/F12, 50% DMEM high glucose, supplemented with 7.5% h.i. FBS and 10 ng/ml EGF, 5 µg/ml insulin, 0.5 µg/ml hydrocortisone, 8.6 ng/ml cholera toxin, 10 µM Y-27632. Add EGF and Y-27632 always fresh to the culture |
| Subculture: | seed out at 1 x 106 cells in a T75 flask; split 1:2 every 6-14 days with Accutase (up to 10 min; wash cells with D-PBS before) and keep at 0.3 -0.6 x 106 in a T75 flask. CAVEAT: cell line has probably limited lifespan, large senescent cells become dominant after about 5 passages; establishment of master and working cell banks is strongly recommended |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 5-14 days |
| Harvest: | maximum density of ca. 0.8 x 105/cm2 |
| Storage: | frozen with RecoveryTM Cell Culture Freezing Medium |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Species validation: | human |
| Cytogenetics: | SNP array indicates chr1q gain, chr10p loss, copy-neutral LOH chr11p |
| Molec. Genetics: | cells lack amplification of MYCN; negative in TRAP assay |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 2080,- € | | Frozen culture | | 1040,- € | | DNA isolated from cell line (25 µg) | | 1300,- € | | DNA isolated from cell line (5 µg) | | 320,- € |
see price list |
| | Print data sheet |
