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Cell line: | CF-10H5 |
DSMZ no.: | ACC 477 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | hybridoma (CD45) |
Origin: | described as secreting a mouse monoclonal antibody (IgG1) CD45; the cell line was established in 1989 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with IL-2-driven T cell lines from patients with high numbers of gamma-delta T cells in the peripheral blood |
Reference(s): | 15170 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single round cells growing in suspension, some loosely adherent |
Medium: | 80% DMEM (4.5 g/L glucose) + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
Subculture: | split saturated culture 1:3 every 1-2 days; seed out at about 0.2 x 106 cells/ml; maintain between 0.2-0.4 x 106 cells/ml; after thawing cells may start slowly to take up growth and may never exceed a viability of 80% |
Incubation: | at 37°C with 10% CO2 |
Doubling time: | about 30-45 hours |
Harvest: | maximal density of about 0.6 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | secretion of a IgG1 monoclonal antibody |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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