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Cell line: | CHP-100 |
DSMZ no.: | ACC 830 |
Species: | human (Homo sapiens) |
Cell type: | Ewing's sarcoma |
Origin: | established from epidural tumor tissue of the spine obtained by thoracic laminectomy of a 12-year old girl with diagnosed neuroepithelioma. Cell line was reported in literature to be tumorigenic in nude mice and in a later analysis erroneously as neuroepithelioma. At DSMZ, it was shown that CHP-100 was misclassified because further analysis demonstrated the chromosomal translocation t(11;22) and the fusion gene product EWS/FLI |
Reference(s): | 18163, 18167, 40348 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently as monolayer; only very few cells exhibit a neuronal-like morphology |
Medium: | 90% McCoy's 5A + 10% h.i. FBS + 2 mM L-glutamine |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2;
split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 36 hours |
Harvest: | cell harvest of 10-15 x 106 cells/80 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | expression of fusion gene EWS-FLI1 was confirmed by RT-PCR |
Viruses: | PCR: HBV -, HCV -, EBV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV + |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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