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| Cell line: | CHP-126 |
| DSMZ no.: | ACC 304 |
| Species: | human (Homo sapiens) |
| Cell type: | neuroblastoma |
| Origin: | established from a large retroperitoneal mass excised from a 14-month-old girl with neuroblastoma (stage III) in 1973; NMYC and DDX1 have been reported to be amplified |
| Reference(s): | 14936 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | spheroidal cells growing as clumps in suspension; grows as semi-adherent monolayer on surfaces coated with poly-L-lysine; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at 2 x 106 cells/80cm2, at best in poly-L-lysine coated flasks; split 1:2 to 1:3 every 3-5 days; resuspend clumps by gentle pipetting on each occasion |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 120-140 hours |
| Harvest: | cell harvest of ca. 8 x 106 cells/80cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype - 46(44-46)<2n>X, -X, +mar, del(1)(p36), t(2;14)(q37;q21-23), dup(7)(p15p22), der(11)t(1;11)(q22;q24), del(12)(q24.32), add(13)(p13), add(14)(p12) - ca. 20-50 dmin - del(1p32-36) and dmin (MYCN amp) are typical of late-stage neuroblastoma |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
