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Cell line: | CI-1 |
DSMZ no.: | ACC 570 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established from the ascites of a 38-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL, lymphoblastic, non-convoluted cell type) at diagnosis in 1981 |
Reference(s): | 14951, 15013 |
Biosafety level: | 1 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | clustered cells in suspension; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; max. density at about 1.5 x 106 cells/ml; cells should be grown in 24-well-plates during the first week after thawing |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human pseudodiploid karyotype with 6% polyploidy - 46(44-49)<2n>XX, t(2;8)(p11;q24), t(14;22)(q32;q11) - matches published karyotype |
Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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