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| Cell line: | CL-34 |
| DSMZ no.: | ACC 520 |
| Species: | human (Homo sapiens) |
| Cell type: | colon carcinoma |
| Origin: | established from a woman with a moderately differentiated primary adenocarcinoma of the right colon (TNM stage 2) |
| Reference(s): | 14528 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing in islets and as monolayer; cells do not grow to complete confluency; image; image |
| Medium: | 80% mixture of DMEM + Ham's F12 at 1:1 + 20% h.i. FBS |
| Subculture: | seed out at ca. 4 x 106 cells/80 cm2; split semi-confluent culture 1:4 to 1:6 once or twice a week using trypsin/EDTA for 10 min; after thawing the use of collagen-coated flasks for the first week is strongly recommended |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells in a 175 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypodiploid karyotype with 16%polyploidy - 45(42-52)<2n>X/XX, -6 - sdl with +3, del(3)(q21) - near-normal "unstable" karyotype with many inconsistent structural and numerical changes |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
