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| Cell line: | CMS |
| DSMZ no.: | ACC 985 |
| Species: | human (Homo sapiens) |
| Cell type: | acute megakaryocytic leukemia |
| Origin: | established in 1991 from a 2-year-old girl with pediatric acute myeloid leukemia (AML M7) at relapse |
| Reference(s): | 40889 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly or in small clumps in suspension; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at about 0.5 x 106 cells/ml and maintain later between 0.3 and 0.5 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | maximal density of 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3-, CD4-, CD13+, CD14-, CD15+, CD19-, CD33+, CD34+, HLA-DR+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
