| | |
|---|
| Cell line: | COLO-679 |
| DSMZ no.: | ACC 264 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established in 1985 from the soft tissue metastasis of a malignant melanoma from a 47-year-old woman |
| Reference(s): | not published |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblastic, but not limited to monolayer growth; image; image |
| Medium: | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture: | split confluent culture 1:5 to 1:10 using trypsin/EDTA; change medium twice weekly; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 34 hours |
| Harvest: | cell harvest of about 25 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human, flat-moded, hypotetraploid karyotype with 8% polyploidy - 83-92<4n>XXXX, -1, +3, -4, +7, +7, -14, -15, -15, -16, -18, -20, -20, +3mar - del(3)(p11)x1-2, del(3)(q13.3), add(7)(q35)x2, del(9)(p22)x2, add(10)(p11)x1-2, add(10)(p13), add(14)(p11), add(19)(p13.3)x2, add(21)(p11), add(22)(q13)x4 - extensive subclonal variation of chs 1, 5 and 7 - 9p- recurrent in melanoma |
| Molec. Genetics: | genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
