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| Cell line: | COLO-783 |
| DSMZ no.: | ACC 257 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established in 1990 from the right axillary lymph node of a 48-year-old woman with metastatic malignant melanoma in the right chest; no direct publication, but genome and proteome analysed; listed in the CCLE (ref 18232) |
| Reference(s): | not published, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblasts, not limited to monolayer growth; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | cells grow very slowly; seed out at about 2-3 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every 1-2 weeks using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 5-7 days |
| Harvest: | cell harvest of about 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 20% polyloidy - 65(61-69)<3n>XXX, -4, -5, -9, -10, -12, +2mar, add(4)(p11), del(6)(p21), del(7)(p14)x2 |
| Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
