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established in 1998 from the pleural effusion of a 35-year-old man with primary effusion lymphoma (PEL) and a history of Kaposi's sarcoma; cells are HHV-8-positive, but HIV-negative. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2.
single round to pleomorph cells growing in suspension; image; image
80-85% RPMI 1640 + 15-20% h.i. FBS
split saturated culture 1:3 every 3-4 days; maintain at ca. 0.5-1.0 x 106 cells/ml; seed out at ca. 1.0 x 106 cells/ml in 24- or 12-well plates; viability may be low after thawing and during the first 1-2 weeks
at 37 °C with 5% CO2
ca. 35-40 hours
maximal density at ca. 1.4 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays