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| Cell line: | CTS |
| DSMZ no.: | ACC 987 |
| Species: | human (Homo sapiens) |
| Cell type: | acute megakaryocytic leukemia |
| Origin: | established in 1982 from peripheral blood of a 13-year-old girl with poorly differentiated megakaryocytic leukemia (AML M1) at relapse after autologous BMT |
| Reference(s): | 40888, 40889 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Distribution to academia only |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out 0.5-1.0 x 106 cells/ml and maintain between 0.5 and 1.5 x 106 cells/ml, split 1:2 to 1:4 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | maximal density of 1.5-2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | CD3-, CD4-, CD7+, CD13+, CD14-, CD15+, CD19-,
CD33+, CD34+, HLA-DR+, cyCD4-, cyCD68-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Molec. Genetics: | expression of fusion gene KMT2A::AFDN (alias MLL::AF6) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
