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Cell line: | Col-GFP-HSC |
DSMZ no.: | ACC 941 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | hepatic stellate cells |
Origin: | established in 2013 from primary hepatic stellate cells (HSC) that were isolated from a transgenic mouse (ref 40441) expressing GFP under control of the collagen alpha1(I) promoter/enhancer; cells were immortalized with SV40 large T; cells are described to be responsive to pro-fibrogenic stimuli |
Reference(s): | 40439, 40440, 40441 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | The cells were isolated from the liver of a transgenic mouse and express GFP under the control of the collagen-alpha1(I)-promoter/enhancer; the cells were immortilized with a plasmid coding for SV40 large T; neither the genetic manipulation of the mouse nor the SV40 large T antigen exhibit human pathogenic properties; the cells were assigned to safety group S1 accordingly German legislation |
Permissions and restrictions: | A, D |
| DSMZ Cell Culture Data: |
Morphology: | fibroblastoid cells growing as monolayer; image; image; image |
Medium: | 90-95% DMEM + 5-10% h.i. FBS + L-glutamine (final concentration 4mM) |
Subculture: | seed out at about 0.5 x 106 cells in a T75 flask; split confluent culture up to 1:40 every 2-4 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 16 hrs |
Harvest: | ca. 8 x 106 cells in a T75 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Molec. Genetics: | expression of GFP and large T antigen was verified |
Viruses: | PCR: MLV + |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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