| | |
|---|
| Cell line: | DERL-2 |
| DSMZ no.: | ACC 531 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell lymphoma |
| Origin: | established from the peripheral blood of a 30-year-old man with hepatosplenic gamma-delta T cell lymphoma (T-cell NHL) in 1995 at disease progression; cells are IL-2 dependent; simultaneous sister cell line of cell line DERL-7 (ACC 524) |
| Reference(s): | 14416, 14460, 14746, 15237 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small multiformed cells growing in large clusters in suspension; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml human IL-2 |
| Subculture: | seed out at ca. 1.0 x 106 cells/ml, cells grow slowly at first, cultivate in 6- or 12-well-plates for optimal growth; a significant amount of cell debris may be present in the culture all the time; split saturated culture 1:2 every 2-3 days; maximal density of ca. 2.5 x 106 cells/ml; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60 hours |
| Harvest: | cell harvest of ca. 2.5 x 106 cells/ml |
| Storage: | frozen in serum-free freezing medium (BambankerTM) |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture and PCR assays |
| Immunology: | CD2 +, CD3 -, CD4 -, CD5 -, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 8% polyploidy - 48(46-49)<2n>XY, +7, +8, +17, t(1;5)(p13;q32), i(7q), der(10;?17)(q10;q10) - sideline with +del(7)(p11) - i(7q) is associated with hepatosplenic T-NHL - matches originator's karyotype |
| Molec. Genetics: | expression of fusion gene CD53::PDGFRB confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
