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Cell line: | DM-3 |
DSMZ no.: | ACC 595 |
Species: | human (Homo sapiens) |
Cell type: | sarcomatoid mesothelioma |
Origin: | established from a pleural biopsy of a 62-year-old man with malignant mesothelioma |
Reference(s): | 14850 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | adherent, sarcomatoid cells growing in monolayers; image; image
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Medium: | 80% DMEM or NCTC-109 + 20% h.i. FBS |
Subculture: | split confluent culture 1:2 to 1:4 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2 |
Incubation: | at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109) |
Doubling time: | ca. 50-60 hours |
Harvest: | cell harvest of ca. 6-8 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with 16% polyploidy;
46(44-48)<2n>XY; no consistent abnormality detected; resembles published karyotype. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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