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| Cell line: | DND-39 |
| DSMZ no.: | ACC 648 |
| Species: | human (Homo sapiens) |
| Cell type: | Burkitt lymphoma |
| Origin: | established from the pleural effusion of a 28-year-old man with American-type EBV-negative Burkitt lymphoma in 1977 |
| Reference(s): | not published |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polygonal cells, growing singly or in small clusters in suspension; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml, optimal split ratio of 1:3 every 2-3 days, maintain at ca. 0.5-1 x 10 6 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-50 hours |
| Harvest: | maximum densitiy of ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10+, CD13-, CD19+, CD20+, CD34-, CD37+, CD38+, CD80(+), CD138-, HLA-DR+,
cyCD79a+, cIgG-, cIgM+, cIg-kappa-, cIg-lambda+, sIgG-, sIgM+, sIg-kappa-, sIg-lambda+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotetraploid karyotype with 4% polyploidy; 86(83-89)<4n>XXYY, -2, -4, -10, -10, -12, -14, -16, -16, +22, +22, der(5)t(2;5)(p24;q14)x2, del(6)(q15)x2 , t(8;14)(q24;q32)x2, add(14)(q32), der(18)add(18)(q21)x2; carries t(8;14) effecting IGH-MYC rearrangement; no reference karyotype available |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
