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Cell line: | EM-3 |
DSMZ no.: | ACC 134 |
Species: | human (Homo sapiens) |
Cell type: | chronic myeloid leukemia in blast crisis |
Origin: | established from the bone marrow of a 5-year-old girl with Philadelphia chromosome-positive CML (chronic myeloid leukemia) in second relapse in 1980 (after bone marrow transplantation day +47); sister cell line of EM-2 (DSM ACC 135) |
Reference(s): | 14488, 14656, 14883 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round to oval cells in suspension; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | maintain at 0.5-1.5 x 106 cells/ml; subculture at a 1:2 to 1:3 dilution every 48 hours; seed out at ca. 0.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40 hours |
Harvest: | grows up to about 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR - |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype - 73(69-77)<3n>X, -X, -X, t(9;22)(q34;q11), i(17q) |
Molec. Genetics: | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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