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| Cell line: | EN |
| DSMZ no.: | ACC 564 |
| Species: | human (Homo sapiens) |
| Cell type: | endometrial carcinoma |
| Origin: | established from the primary endometrial (cervical) carcinoma (stage IIIC) which was histologicall poorly differentiated from a 50-year-old woman prior to therapy |
| Reference(s): | 14630 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epitheloid cells growing in monolayers; image; image; image; image |
| Medium: | 80% DMEM + 20% h.i. FBS |
| Subculture: | split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out 1.5 x 106 cells in 80 cm2; maintain at 0.1-0.2 x 105 cells/cm2; after recovery from N2 cell growth is slow (doubling time > 120 h) |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 50-80 hours |
| Harvest: | cell harvest of ca. 1 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded near-tetraploid karyotype with 3% polyploidy - 79-97<4n>XXX/XXXX, -13, +2mar, 0-4 dm, der(1;7)(q10;q10)x2 - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
