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Cell line: | ESO-26 |
DSMZ no.: | ACC 708 |
Species: | human (Homo sapiens) |
Cell type: | esophageal adenocarcinoma |
Origin: | established from the primary tumor (pT4N1M1) of a 56-year-old man with adenocarcinoma of the esophagus in 2000; cells are described in the literature to carry a p53 mutation (C742T); whole genome sequence data in ref 40877 |
Reference(s): | 16055, 40877 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | ovoid cells growing semiadherently, singly or in clumps
image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 2-5 x 106 cells/175 cm2 flask; split culture 1:2 to 1-6 every 2-6 days using trypsin/EDTA or possibly by tapping the flask |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35-50 hours |
Harvest: | cell harvest of ca. 100 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypodiploid karyotype; 39(36-40)<2n>X, -Y, +2, -3, -5, -11, -12, -15, -16, -19, -20, -22, +mar
add(X)(p22), del(1)(p32), add(12)(p13), der(13;21)(q10;q10), i(13)(q10), add(17)(p12), add(?18)(q21), add(21)(p11) |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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