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Cell line: | ESO-51 |
DSMZ no.: | ACC 694 |
Species: | human (Homo sapiens) |
Cell type: | esophageal adenocarcinoma |
Origin: | established in 2000 from the primary tumor of a 74-year-old white man with adenocarcinoma of the esophagus; whole genome sequence data in ref 40877 |
Reference(s): | 16055, 40877 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | polymorphic cells growing in clusters in suspension |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.1-0.2 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; cell clusters are difficult to disperse and cell counting may be difficult; viability may drop below 60% after recovery from liquid nitrogen, but cells recover after 4-5 days |
Incubation: | at 37 °C with 5% CO2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat bimodal hyperdiploid/hypertetraploid karyotype; 51-63/90-121<4n>XXXX, -Y, -Y, -Y, -Y, + 5-12mar; add(X)(p11)x2, del(2)(q21)x2, dup(2)(q11q24)i(2)(q10), dup(3)(q24q29), i(3)(p10), add(3)(p25), add(7)(q21)(hsr)x2, del(13)(q11q13), add(17)(q 25), add(21)(p11) |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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