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| Cell line: | ESS-1 |
| DSMZ no.: | ACC 461 |
| Species: | human (Homo sapiens) |
| Cell type: | endometrial stromal sarcoma |
| Origin: | established from an endometrial stromal sarcoma of the uterus of a 76-year-old woman; cells were described as demonstrating a mainly mesenchymal phenotype with signs of epithelial characteristics within foci and an extensively multilayered growth pattern |
| Reference(s): | 14582, 14546 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells growing in monolayers, partially also in multilayers; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 every week using trypsin/EDTA, but change the medium more frequently; maximum density of ca. 10-15 x 106 cells/175 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 120 hours |
| Harvest: | cell harvest of ca.10 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human karyotype with hyper-diploid/tetraploid and -octaploid clones present in approx. equal numbers - 47(45-47)<2n>XX, +19, der(3)t(3;6)(q2?8;p11-12), add(6)(q27), add(19)(p11) - sideline with i(2q) - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
