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| Cell line: | FKH-1 |
| DSMZ no.: | ACC 614 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myelocytic leukemia |
| Origin: | established in 1993 from the peripheral blood of a 61-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) with trilineage myelodysplasia at refractory leukemic transformation into acute myeloid leukemia (AML M4); carries t(6;9)(p23;q34) leading to DEK-NUP214 (DEK-CAN) fusion gene; contrary to what was originally described the cells do not respond to G-CSF |
| Reference(s): | 14594 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly single, round cells in suspension; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1.0 x 106 cells/ml in 24- or 12-well plates; optimal split ratio of 1:2 or less every 3-4 days; cultures may contain a high amount of cell debris; the accumulation of cell debris can be prevented by using new culture vessels every second week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-100 hours |
| Harvest: | maximum density at ca. 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4+, CD11b+, CD13+, CD14-, CD15(+), CD19-, CD33+, CD34-, HLA-DR(+),
cyCD3+, cyCD68+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 4% polyploidy; 45(43-46)<2n>XY, -7, t(6;9)(p23;q34);
carries t(6;9) effecting rearrangement of DEK with NUP214 (CAN); closely resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
