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| Cell line: | GH4-C1 |
| DSMZ no.: | ACC 482 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | pituitary tumor |
| Origin: | subclone of the cell line GH3 (ACC 469); established from a pituitary tumor induced in a female Wistar/Furth rat in 1965; described in the literature to produce a high rate of prolactin and a low rate of growth hormone synthesis |
| Reference(s): | 14880, 15008, 15009, 15004, 15194 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly round cells growing singly and in clusters in suspension and partially adherent; quality and amount of serum may influence the adherence of the cells; image; image; image |
| Medium: | 80-90% Ham's F10 + 10-20% h.i. FBS + 2 mM L-glutamine (originally grown with 15% horse serum + 2.5% h.i. FBS in Ham´s F10) |
| Subculture: | cells grow slowly and do not reach high cell densities; seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-0.8 x 106 cells/ml; adherent cells might be detached by "washing" or with TNE (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl); |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-80 hours |
| Harvest: | maximal density of 0.8 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat karyotype |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
